Journal «MEDICINA» 2/2021

Lens opacity, which leads to cataract, is one of the most complicated issues in ophthalmology. Pathophysiology of cataract progression is far from being clearly established. Despite low lipid content in the lens of the eye, there is an assumption that they and their predecessors may be involved in the development of this disease. Given the many roles of monounsaturated fatty acids, it can be expected that variations in stearoyl-Coa desaturase activity will affect a number of key physiological processes.

Key words: δ9 desaturases, stearoyl-Coa desaturase, cataract, lipid metabolism, eye lens

The current state and user audience of the website of the largest drug treatment clinic in the Russian Federation is analyzed. The possibilities of monitoring and analyzing the activity of project users by means of the existing network tools are described. Conclusions are made about the need for the earliest possible introduction of means for contactless communication between employees of medical organizations and patients, as well as the integration of the website into the structure of information flows of the organization. The question is raised about the need to investigate the correspondence between the sociodemographic characteristics of the audience of the website and the consumers of the clinic's services.

Key words: digital technologies, medical information, website audience, information flows, telemedicine

The article examines the results of a three-year monitoring of public opinion on public awareness about measures to reduce the prevalence of tobacco consumption in the Russian Federation, which was conducted on the basis of Internet surveys (SLOP research) using a questionnaire developed in accordance with the objectives of the study in 2019-2021 years. The questionnaire also included questions about the consumption of tobacco and nicotine-containing products. The purpose of the monitoring was to identify existing and emerging trends in the consumption of tobacco and nicotine, in the awareness of the population and information sources. This article describes the study of the dynamics of consumption of tobacco and nicotine-containing products among the survey respondents, as well as analyzes the preferences in the consumption of these products and their combinations depending on age and gender.

Key words: tobacco, nicotine, consumption of tobacco products, consumption of nicotine-containing products, dynamics of tobacco consumption in the Russian Federation, monitoring of tobacco consumption, survey on tobacco consumption, consumption of tobacco and nicotine among young people, consumption of electronic cigarettes, consumption of vapes

Introduction. Described at the beginning of HIV epidemics in the USA syndrome (or syndemia) of substance abuse, violence and AIDS (SAVA) still continue to be an important risk factor for HIV-infection acquisition. The goal of this study was to analyze association between SAVA and STI and also new cases of HIV infection among IDU in six cities in Russian Federation. Materials and methods. Analysis is based on the data from bio-behavioral survey conducted in six Russian cities with help of respondent-driven sampling. Results. It was found no statistically significant association between SAVA and STI after correction for sampling, but without correction STI prevalence in group without SAVA was 4.1% and in group with SAVA and binge drinking – 9,2% (р=0.04). Stratified analysis showed that it is impossible to combine data from males and females and in case of separate analysis statistically significant association between SAVA and STI exists in females (р=0,027). Analysis with adjustment for interview site, gender and age SAVA with binge drinking significantly elevates STI risk (OR=2.69 [95%CI=1.21... 5.99], р=0.016). Study of association between SAVA components and new cases of HIV found that experience of physical and/or sexual violence combined with binge drinking increase risk of HIV acquisition (adjusted for interview site, gender, age, needle/syringe sharing, education and sex work OR=4.03 [95%CI=1.19...13.69], р=0.026). Experience of physical and/or sexual violence combined with binge drinking was significantly associated with such HIV risk factors as needle and syringe sharing (OR=3.07 [95%CI=1.02...9.24], p=0.046) and sex work (OR=17.29 [95%CI=3.83...77.96], p<0.001). Conclusion. SAVA existence should be taken into account when planning preventive measures in Russian Federation and existing programs of comprehensive prevention should have components designed to decrease consequences of syndemia.

Key words: syndemics, SAVA syndrome, IDU, violence, HIV-infection

A retrospective analysis of case histories and the results of microbiological blood tests of patients with coronavirus infection COVID-19 who were treated in the infectious diseases hospital of the city clinical hospital №52 in Moscow in 2020 was carried out. The sensitivity to the main antibacterial agents was determined for each isolate of Klebsiella pneumoniae, the phenotype of each pathogen was characterized (MDR, XDR, PDR). The results of the study were statistically processed and presented in the form of a register of bacteremia cases. Most pathogens (79%) were isolated from the blood of patients who were treated in intensive care units. Klebsiella pneumoniae ranked first in the structure of bacteremias, the frequency of occurrence of the pathogen was 41,1% (282/686). Only 4% of Klebsiella pneumoniae isolates were sensitive to antibiotics; the phenotypes of antibiotic-resistant strains were MDR – 16%, XDR – 76%, PDR – 4%. Most of the isolated Klebsiella pneumoniae strains were carbapenem-resistant (85,4%), 11,1% were colistin-resistant, 10,6% of the isolates were extended spectrum β-lactamase producers.

Key words: bacteremia, antibiotic resistance, Klebsiella pneumoniae, COVID-19

A technique has been developed based on reversed-phase high-performance liquid chromatography with diode-matrix detection for the joint determination of succinic acid and cetylpyridinium chloride in complex action medicinal films. Efficient chromatographic separation of active drug components was achieved in a gradient elution mode on a Luna C18 (2) 100A column (4.6 × 250 mm, 5 µm) using a mobile phase consisting of a 0.1% solution of phosphoric acid and acetonitrile. The detection wavelength was 210 nm for both compounds. The developed method is validated in terms of specificity, linearity, precision, accuracy and can be used to determine the authenticity and quantitative content of succinic acid and cetylpyridinium chloride in the joint presence in assessing the quality of medicinal films.

Key words: medicinal films, succinic acid, cetylpyridinium chloride, high performance liquid chromatography, gradient elution

The aim of the study was to analyze the attitude of the population to HIV testing and counseling and the frequency of testing-related counseling in a sample of the population of Kazan. Materials. An anonymous survey of various groups of the population permanently residing in the city of Kazan (n=301) was conducted using a questionnaire developed by us. The study involved 58.1% of women (175/301), 41.9% of men (126/301). Results. Most of the respondents had experience of undergoing HIV testing (95%, 286/301). Only 92 people out of 286 (32.2%) were examined on their own initiative. HIV testing was mainly carried out in the polyclinic at the place of residence/stay (66.8%, 191/286), while counseling, according to respondents, was carried out only in 16.20% of respondents (31/191). Conclusions. It is important to conduct a high-quality procedure of pre-and post-test counseling in the conditions of primary health care. It is necessary to train the medical staff of the polyclinic level in the skills of counseling on epidemiology and prevention of HIV infection.

Key words: HIV infections, testing, counseling

The use of multiply regression analysis for the TB indicators assessment and prediction in the Republic of Crimea is discussed. Basic TB indicators in the Republic of Crimea for the period 2014-2018 were studied using Pearson correlation coefficient and step-by-step multiply linear regression analysis. During paired correlation analysis, dependable association with an average and high degree is identified for the most indicators of interest. The multiple correlation coefficients were higher than the paired correlation coefficients, which demonstrated the greater significance of the group conditionality of the indicators. For key epidemiological TB indicators the regression equations are constructed and the most relevant predictors are found using stepwise multiply regression analysis. The regression equations for TB incidence, prevalence and mortality rates are proposed for practical applying to predict epidemiological situation.

Key words: tuberculosis, indicators, epidemiologic situation, correlation analysis, multiply regression analysis, forecasting, Republic of Crimea