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Preliminary results of a study based on genome-wide sequencing of resistant K.pneumoniae strains in a multidisciplinary hospital in Barnaul

Journal «MEDICINA» ¹ 4, 2023, pp.42-54 (Research)


Russkih A. A.
Postgraduate student, Chair for Epidemiology, Microbiology and Virology1

Luk'janenko N. V.
Doctor of Medicine, Professor, Chair for Epidemiology, Microbiology and Virology1

Rudenko A. V.
Head, Educational laboratory, Lecturer1

Kolomiets A. A.
Vice-Rector for Regional Healthcare Development1

Petrova A. A.
Head, Epidemiological Department2

Mikhailova Y. V.
PhD (Biology), Head, Laboratory of Molecular Mechanisms of Antibiotic Resistance3

1 - Altai State Medical University, Barnaul, Russian Federation
2 - Regional Clinical Hospital, Barnaul, Russian Federation
3 - Central Research Institute of Epidemiology, Moscow, Russian Federation

Corresponding Author

Russkih Artjom Aleksandrovich; e-mail: Likvidator20110@mail.ru

Conflict of interest

None declared.


The study had no sponsorship.


Introduction. Klebsiella pneumoniae, one of the leading causative agents of nosocomial infections, is included in the group of so-called «ESKAPE» pathogens – microorganisms associated with increased antibiotic resistance, representing a serious problem for public health. K. pneumoniae is widespread and has a pronounced ability to acquire resistance to antimicrobial drugs. Inadequate antibacterial therapy in patients with nosocomial infections worsens the prognosis of the disease and increases hospital mortality, therefore, the prescribing of medications should be based on knowledge of the current profile of antibiotic resistance of the pathogen in a particular hospital. The study of the genetic diversity of K. pneumoniae will reveal the main mechanisms of resistance of this pathogen and formulate recommendations for rational antibiotic therapy. Objective. Genome-wide analysis of resistant K. pneumoniae isolates in the context of epidemiological surveillance of healthcare associated infections (HAI) in a multidisciplinary hospital. Materials and methods of research. 41 isolates of bacteria of the genus Klebsiella isolated in a multidisciplinary hospital in Barnaul from clinical samples were studied. Genome-wide sequencing was performed using NextSeq 2000 (Illumina). Research results and their discussion. A comparative analysis of the microbiological background in a multidisciplinary hospital and hospitals in Russia demonstrated the predominance of K. pneumonia in the overall structure: 39.2% and 27.86%. In the course of genome-wide analysis of resistant strains of K. pneumoniae, combinations of beta-lactamase genes and genes encoding mechanisms of resistance to disinfectants were identified. Genes associated with the resistance of Klebsiella pneumoniae to hydrogen peroxide were found, genes of carbapenemases of the OXA-48 group, genes of extended-spectrum beta-lactamases of the CTX-M group, as well as metallo-b-lactamases of the NDM group were identified among the studied cultures. Conclusion. The use of genome-wide sequencing of isolates of HAI pathogens in clinical practice (using the example of Klebsiella pneumoniae) determines the choice of antibacterial therapy, the use of disinfectants and antiseptics in the organization of preventive and antiepidemic measures.

Key words

Klebsiella pneumoniae, antibiotic resistance, genome-wide sequencing



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