Authors
Bykov Yu. V.
MD, PhD, Assistant, Chair for Anesthesiology, Resuscitation and Emergency Medicine1
Baturin V. A.
Doctor of Medicine, Professor, Head, Chair for Clinical Pharmacology1
Vorobyeva A. P.
MD, Anesthesiologist-Resuscitator2
Vartanyan A. A.
MD, Junior Researcher, ì.í.ñ., Laboratory of Pharmacology of the Center for Morphology, Pharmacology and Biotechnology; Resident Physician, Chair for Clinical Laboratory Diagnostics1
1 - Stavropol State Medical University, 355017, Stavropol, Russian Federation
2 - G.K. Filippsky City Children's Clinical Hospital, Stavropol, Russian Federation
Corresponding Author
Vorobyova Anna; e-mail: a.v.955@yandex.ru.
Conflict of interest
None declared.
Funding
The study had no sponsorship.
Abstract
Background. Type 1 diabetes mellitus (DM) is one of the most prevalent chronic diseases in children. Oxidative stress (OS) plays an important role in the development of diabetes and its complications. Aim of the study. To analyze markers of OS in children with newly diagnosed and chronic course of type 1 DM. Materials and methods. The study included 73 children aged 3 to 17 years. The study group consisted of 49 patients with type 1 DM, the control group included 24 children (apparently healthy children). The study group was split into 2 subgroups depending on the duration of the disease: 1 subgroup – children with newly diagnosed type 1 DM, 2 subgroup – children with a chronic course of DM. All children had their blood sampled to assess the following OS markers: 8-Oxo-2'-deoxyguanosine (8-OHdG; Pg/ml); 8-isoprostane (8 IS; Pg/ml); malondialdehyde (MDA; Ng/ml); advanced oxidation protein products (AOPPx10; Ng/ml). Statistical processing of data involved the use of the Mann–Whitney U test and the Kruskal–Wallis test. Results. Significant differences in the average values of OS markers between patients with type 1 DM and control group were revealed. The average levels of 8 IS – 89.53 [81.96-106.4] Pg/ml were significantly higher in the study group, compared with the control – 78.21500 [65.235-88.135] Pg/ml (p=0.0006), as were the average levels of 8-OHdG – 480.3 [413.8000-560.3000] Pg/ml in the study group; 417,9000 [361,7500-460.7] Pg/ml – in the control group (p=0.01). The maximum values were found in the levels of 8 IS and 8-OHdG, in the group of children with chronic type 1 diabetes: 510.1 [440.9-594.4] Pg/ml (p =0.0053) for 8-OHdG and 95.59 [86.32-197.5] Pg/ml (p=0.0001) for 8 IS. The average levels of MDA and AOPP did not show significant differences between the study and the control groups. Conclusion. Patients with type 1 DM have distinct manifestations of OS. These manifestations are most pronounced in the group of patients with chronic type 1 DM.
Key words
type 1 diabetes mellitus, oxidative stress, children and adolescents, isoprostane, malondialdehyde
DOI
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