Standardization of Dry Extract of Pumpkin Pulp Juice, Which Has a Hypolipidemic Effect on the Content of Β-Carotene

Landina L. N.
Postgraduate, Chair for Pharmacology with a course in Clinical Pharmacology¹

1 - Pyatigorsk Medical Pharmaceutical Institute of Volgograd Medical State University of the Ministry of Healthcare of Russia

Landina Ludmila; e-mail: llandina@mail.ru

None declared.

The study had no sponsorship.

The objective is to standardize the content of β-carotene – one of the principal active substances with sufficient hypolipidemic activity in the resultant original product. Search for natural biologically active substances having a hypolipidemic effect is one of the topical questions in modern pharmacy. Most of the research is carried out by means of isolating individual substances from the plant object. We propose to study a native phytocomplex obtained from pumpkin pulp – a dry extract of pumpkin pulp juice. The following materials and methods were used: laboratory-obtained dry extract of pumpkin pulp juice and methods used to determine the qualitative and quantitative content of β-carotene in an individual substance. It was possible to adapt the methods applied to the analysis of an individual substance in relation to the resulting phytocomplex containing both lipophilic and hydrophilic fractions. The rapid method of quantitative determination of β-carotene described in the scientific literature was corrected. An effective alternative method for spectrophotometric determination of β-carotene in the phytocomplex has been developed. Phytocomplexes standardized for the main active substances can be used to obtain effective drugs and biologically active additives with minimal side effects. In addition, the expansion of the search for alternative natural objects containing complexes haaving a hypolipidemic effect is currently relevant. Based on the literature data, in the future it is planned to conduct a pharmacological study to establish the anti-inflammatory effect of the resulting complex on the state of the vascular wall.

isolation and individualization, native phytocomplex, standardization of the active substance, special system of solvents, spectrophotometer

10.29234/2308-9113-2021-9-1-79-92

1. Alexashina S.A., Makarova N.V. Investigation of the chemical composition and the antioxidant activity of carrots, beets and pumpkin [Investigation of the chemical composition and the antioxidant activity of carrots, beets and pumpkin]. Hranenie i pererabotka sel«hozsyr»ja [Storage and processing of farm products] 2016; 6: 29-32. (In Russ.)

2. Vasilenko Ju.K., Klimova O.V., Landin V.V. Gipolipidemicheskaja aktivnost' morkovnogo i tykvennogo sokov. Strukturno-funkcional'nye vzaimodejstvija pri kompensacii narushennyh funkcij. Proceeding of UNESCO medical center «Unona» 2000; 4: 95-96. (In Russ.)

3. Vishnevskaja L.I., Degtjareva E.A., Bisaga E.I., Tkachuk O.Ju. Issledovanie himicheskogo sostava biologicheski aktivnyh veshhestv v lipofil'nom jekstrakte tykvy. Himija rastitel'nogo syr'ja 2014; 3: 167-170. (In Russ.)

4. Golobkina N.A., Pyshnaja O.N., Bondareva N.V. Biologicheskoe znachenie karotinoidov [Biological role of carotenoids]. Ovoshhi Rossii [Vegetable crops of russia] 2010; 2: 26-40. (In Russ.)

5. Gorbatjuk N.O., Chernikov M.V., Dodohova M.A., Terehov A.Ju., Rekkandt S.A., Mashirova S.Ju. Vlijanie summy triterpenovyh kislot oblepihi (Hippopha rhamnoides l.) i kljukvy (Vaccinium oxycoccos l.) na pokazateli lipidnogo i uglevodnogo obmenov pri kursovom vvedenii zdorovym zhivotnym. Uspehi sovremennoj nauki i obrazovanija 2017; 1(1): 55-59. (In Russ.)

6. Dawson R., Elliott D., Elliott U., Jones K. Spravochnik biohimika. Moscow: Mir, 1991. 424 p. (In Russ.)

7. Evdokimova O.V., Samylina I.A., Nesterova O.V. Opredelenie soderzhanija summy fosfolipidov i karotinoidov v plodah nekotoryh vidov bojaryshnika. Farmacija [Farmatsiya] 1992; 41(6): 70-72. (In Russ.)

8. Zavyalova T.I., Kostko I.G. Biologicheskaya tsennost' tykvy i produktov ee pererabotki [The biological value of fresh and processed pumpkin fruit]. Izvestija Sankt-Peterburgskogo gosudarstvennogo agrarnogo universiteta [Izvesniya Saint-Petersburg state agrarian university] 2015; 39: 45-58. (In Russ.)

9. Kuregyan A.G. Izuchenie karotinoidov tykvy metodami spektrofotometrii i tonkosloynoy khromatografii [Study carotenoids pumpkin by spectrophotometry and TLC]. Sovremennye problemy nauki i obrazovanija [Modern problems of science and education. Surgery] 2015; 1-2: 231. (In Russ.)

10. Landina L.N., Landin V.V. Tekhnologiya sukhogo ekstrakta soka myakoti tykvy kak perspektivnogo ob«ekta pri sozdanii preparata, obladayushchego gipolipidemicheskoy aktivnost'yu [The technology of dry extract juice pumpkin pulp as a promising object to create the drug, which has hypolipidemic activity]. Razrabotka, issledovanie i marketing novoj farmacevticheskoj produkcii [Development, research and marketing of the new pharmaceutical products] 2017; 72: 113-115. (In Russ.)

11. Razhabova G.H., Karomatov M.D., Hoshimova N. Tykva kak lechebnoe rastenie i perspektivy ego primeneniya v klinike vnutrennikh bolezney [Pumpkin as the medical plant and the prospects of its application in clinic of internal diseases]. Biologija i integrativnaja medicina [Biology and iintegrative medicine] 2017; 3: 144-155. (In Russ.)

12. Bryon A., Kurlovs A.H., Dermauw W., Greenhalgh R., Riga M., Grbic M., Tirry L., Osakabe M., Vontas J., Clark R.M., Van Leeuwen T. Disruption of a horizontally transferred phytoene desaturase abolishes carotenoid accumulation and diapause in Tetranychus urticae. Proc Natl Acad Sci USA 2017; 114(29): 5871-5880.

13. Maoka T. Carotenoids as natural functional pigments. Journal of Natural Medicines 2020; 74(1): 1-16.

14. Melendez-Martinez A.J., Mapelli-Brahm P., Benitez-Gonzalez A., Stinco C.M. A comprehensive review on the colorless carotenoids phytoene and phytofluene. Archives of Biochemistry and Biophysics 2015; 572: 188-200.

15. Nishino A., Ichihara T., Takaha T., Kuriki T., Nihei H., Kawamoto K., Yasui H., Maoka T. Accumulation of paptika carotenoid in human plasma and erythrocytes. J Oleo Sci 2015; 64(10): 1135-1142.

16. Priyadarshani A.M.B. A review on factors influencing bioaccessibility and bioefficacy of carotenoids. Critical Reviews in Food Science and Nutrition 2017; 57(8): 1710-1717.

17. Rivera S., Vilaró F., Canela R. Determination of carotenoids by liquid chromatography/mass spectrometry: effect of several dopants. Analytical and Bioanalytical Chemistry 2011; 400: 1339-1346.

18. Stincoa C.M., Benítez-Gonzáleza A.M., Hernanzb D., Vicarioa I.M., Meléndez-Martíneza A.J. Development and validation of a rapid resolution liquid chromatography method for the screening of dietary plant isoprenoids: carotenoids, tocopherols and chlorophylls. Journal of Chromatography A 2014; 1370: 162-170.

19. Ying Wang, Sang-Jin Chung, Marjorie L. McCullough, Won O. Song, Luz Fernandez M., Sung I. Koo, Ock K. Dietary carotenoids are associated with cardiovascular disease risk biomarkers nediated by serum carotenoid concentrations. Journal of Nutrition 2014; 144(7): 1067-1074.