Evaluation of diagnostic efficiency of preliminary immunochromatographic analysis during chemical-toxicological studies on synthetic cathinones

Grebenkina E. V.
¹

Gavryushov S. A.
¹

Lisovskaya S. B.
¹

Kardonsky D. A.
¹

1 - FSAEI HE I.M. Sechenov First MSMU MOH Russia (Sechenovskiy University)
119991, Moscow, Trubetskaya Str. 8

The article presents a method for assessing the diagnostic effectiveness of immunochromatographic analysis in hardware-software execution during chemical-toxicological studies and establishing the level of "cutoff" with the probability of detection of synthetic cathinones in persons directed to medical examination of establishment intoxications. New immunochromatographic test systems at the stage of screening can detect synthetic cathinones in the urine in the range from 20 ng/ml. The overall accuracy of the test was 92.3%. Therefore, to confirm a positive result obtained during screening, on the second stage of chemical-toxicological researches, it is necessary to have a reliable method for detecting analytes in the urine when they are found at low concentrations. Such methods include gas chromatography with mass-selective detection (GC-MS).

synthetic cathinones, immunochromatographic analysis, sensitivity, specificity, ROC-analysis

10.29234/2308-9113-2018-6-3-140-152

1. Asadullin, A. R., Akhmetov E. A., Nenasheva A. Y. Katinony. Novaya real'nost' [Cathinone. New reality]. Narkologiya [Narcology] 2017; (1): 87-92. (in Russ.)

2. Burtsev A. A., Iskandarov, R., Smirnov A.V., Petuhov A.V., A. Y. Nenasheva Nekotorye aktual'nye problemy monitoringa novyh psihoaktivnyh veshchestv v Moskve [Some of the current problems of monitoring new psychoactive substances in Moscow] Narkologiya [Narcology] 2018; (6): 53-59. (in Russ.)

3. Golovko A. I., I. V. And Ivanov M. B., Barinov V. A, E. Y. Bonitenko Dizajnerskie narkotiki. Klassifikacii, mekhanizmy toksichnosti [Designer drugs. Classifications, mechanisms of toxicity]. Narkologiya [Narcology] 2015; (8): 69-85. (in Russ.)

4. GOST R ISO 18113.1-2015 Medicinskie izdeliya dlya diagnostiki in vitro. Informaciya, predostavlyaemaya izgotovitelem (markirovka) Chast' 1 [State Standard R ISO 18113.1-2015 Medical devices for in vitro diagnostics. The information provided by the manufacturer (labelling) Part 1]. (in Russ.)

5. GOST R 51352-2013. Medicinskie izdeliya dlya diagnostiki in vitro. Metody ispytanij. [State Standard R 51352-2013. Medical devices for in vitro diagnostics. Test method]. (in Russ)

6. GOST R 53022.2-2008 Tekhnologii laboratornye klinicheskie. Trebovaniya k kachestvu klinicheskih laboratornyh issledovanij (tochnost', chuvstvitel'nost', specifichnost') [State Standard R 53022.2-2008. Clinical laboratory technologies. Quality requirements for clinical laboratory tests (accuracy, sensitivity, specificity)]. (in Russ.)

7. Larchenko A.V., Suvorov M.A., Andryuhin V.I. et al. Sinteticheskie katinony i kannabinoidy – novye psihoaktivnye veshchestva (obzor) [Synthetic cathinones and cannabinoids – new psychoactive substances (review)]. Sovremennye tekhnologii v medicine [Modern technologies in healthcare] 2017; 9(1):185-186. (in Russ.)

8. Moskaleva E.V., Eroshchenko N.N., Kiryushin A.N. et al. Obnaruzhenie al'fa-pirrolidinovalerofenona (al'fa-PVP) i ego metabolitov v ob"ektah sudebno-himicheskogo issledovaniya [Detection of alpha-pyrrolidinovalerophenone (alpha-PVP) and its metabolites in the objects of forensic chemical research]. Sud.-med. ehks. [Forensic Expertize] 2017; 60(1):19-23. (in Russ.)

9. Nadezhdin A.V. Vliyanie vozrastnogo faktora na kliniku narkologicheskih zabolevanij [Influence of age factor on the clinic substance abuse and drug addiction] in Narkologiya [Narcology] 2016; (5): 90-99 (in Russ.)

10. Rukovodstvo po ehkspluatacii analizatora dlya himiko-toksikologicheskih issledovanij IK 200609 [Analyzer operating manual for chemical and Toxicological studies IK 200609], T & D Innovationen GmbH (2016) (in Russ.)

11. Silant'ev A.S., Grebenkina E.V. Nosyrev A.E. Neobhodimost' vvedeniya metodov kolichestvennoj ocenki psihoaktivnyh veshchestv v biologicheskih ob"ektah [The need to introduce methods for the quantitative evaluation of psychoactive substances in biological objects]. Vopr. nark. [Journal of Addiction Problems] 2018; (1):138-152. (in Russ.)

12. Yalovega A.V. et al. Himiko-toksikologicheskaya laboratoriya kak instrument monitoringa narkoticheskih sredstv, psihotropnyh veshchestv na territorii respubliki Bashkortostan. Problemy zloupotrebleniya lekarstvennymi preparatami i novymi psihoaktivnymi veshchestvami: sb. mezhd. nauch.-praktich. konf. (Perm', 12-14 maya 2016 g) [Chemical toxicology laboratory as a tool for monitoring narcotic drugs, psychotropic substances in the territory of the Republic of Bashkortostan. Problemy zloupotrebleniya lekarstvennymi preparatami i novymi psiho-aktivnymi veshchestvami: sb. mezhd. nauch.-praktich. konf. (Perm', 12-14 May 2016)]. P. 47-52. (in Russ.)