Review of Spectrofluorimetric Studies of Skin

Gar’kavenko V. V.
PhD, Department Head¹

Balashova P. M.
Ophthalmologist¹; Assistant, Department of Ophthalmology named after Professor M. A. Dmitriev²

Salmin V. V.
Doctor of Physics and Mathematics, Professor, Chair for Laser Micro- Nano- and Biotechnologies³; Professor, Chair for General Physics⁴; Professor, Chair for Physics⁵

1 - Professor P. G. Makarov Krasnoyarsk Regional Ophthalmological Clinical Hospital. Krasnoyarsk, Russian Federation
2 - Professor V. F. Voino-Yasenetsky Krasnoyarsk State Medical University. Krasnoyarsk, Russian Federation.
3 - National Research Nuclear University (Moscow Engineering Physics Institute), Moscow, Russian Federation
4 - Moscow Institute of Physics and Technology (National Research University), Dolgoprudny, Russian Federation
5 - Bauman Moscow State Technical University. Moscow, Russian Federation

Gar’kavenko Victor Valerievich; e-mail: victor-unique@yandex.ru

None declared.

The study had no sponsorship.

Background. Nicotinamide adenine dinucleotide (NADN) is involved in many important biochemical reactions in human metabolism, including participation in mitochondrial energy production. Mitochondrial activity, which affects skin microcirculation and the efficiency of local blood supply, can be indirectly assessed by the fluorescence of NADH in its reduced form. Changes in major skin fluorophores have been studied in both animals and humans, at rest and under different conditions, using spectrofluorimetry. The purpose of the work is, based on scientific literature data, to evaluate the possibility of using a spectrofluorimetric study of NADH levels, as well as the NADH/FAD ratio when assessing the condition of both free skin flaps and pedicled flaps after reconstructive operations, in particular in ophthalmology. Materials and methods. A search of scientific publications in PubMed, Google Scolar and Elibrary was conducted, as a result 61 articles for the period of 1957-2022 were selected for the final scientific review. Results and discussion. A brief historical background of the first spectrofluorimetric studies is presented. A brief overview of spectrofluorimetric methods for studying the skin of both humans and experimental animals, as well as other organs and systems, is given. The disadvantages and advantages of various research methods are listed. A review of changes in spectrofluorimetric parameters of the skin in patients with various somatic diseases of organs and systems was carried out. Conclusion. A review of the abovementioned methods for assessing tissue metabolism is applicable in surgical treatment, plastic surgery and onco-ophthalmology. Of particular value for surgery will be research methods that make it possible to predict the likelihood of rejection of transplanted skin flaps and their cicatricial changes. The presented literature review confirms the effectiveness of spectrofluorimetry for assessing metabolic disorders in the skin. The relevance of the method for predicting cosmetic outcomes during plastic surgery, including on the adnexal apparatus of the eye, requires continued research into the main fluorophores of the skin.

cicatricial changes of the skin of the eyelids; NADH; skin; spectrofluorimetry, autofluorescence

10.29234/2308-9113-2024-12-3-69-80

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